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A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.)

Nguyen Thi Thuy Linh, Pham Thi Ngoc, Ninh Thi Thao, Nguyen Thi Phuong Thao

Received: Feb 15, 2019 / Published: Feb 16, 2019
This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA. The highest shoot multiplication rate (9.57 times) was obtained on MS medium containing 1 mg L-1 BA and 0.25 mg L-1 a-NAA after 8 weeks of culture. 100% of the shoots produced roots with a mean of 10.10 roots per plant within 4 weeks on ½ MSM medium with 4 mg L-1 IBA. The survival rate of in vitro derived plantlets after a 6 to 7-week-period of rooting during acclimatization using a soil: coco peat: smoked rice husks (2:2:1, v/v/v) substrate was 100% and acclimatized plantlets showed good growth and development. This is the first report on a practical and efficient in vitro multiplication protocol for Japanese cherry in Vietnam, starting from shoot initiation to establishment of plants under greenhouse conditions.
Establishment of Reciprocal Micrografting of Tomato (Solanum lycopersicum L.) and Eggplant (Solanum melongena L.)

Son Truong Dinh, Tham Thi Tran

Received: Oct 19, 2018 / Accepted: Oct 22, 2018 / Published: Oct 22, 2018
Micrografting can be used as a key tool to investigate gene-function, long-distance signal transduction, or metabolite movement in different developmental and physiological stages. In plant production, plant grafting can be applied to improve productivity and/or increase the tolerance of plants to stresses. Here, we describe a simple and high efficiency protocol for reciprocal micrografting of tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.). Tomato and eggplant seeds can be disinfected with 0.5% Presept for 20 min before germinating on MS media. Seedlings of 5-day-old tomatoes and 15-day-old eggplants were suitable for preparation of scions and rootstocks. Scions were cut into 0.5-1 cm lengths for micrografting. Sucrose levels greatly influenced the graft success rate of all graft combinations including of self- and reciprocal micrografting between tomato and eggplant. While self-grafted tomatoes or eggplants required 20 g L-1 sucrose to get the highest grafting success rate (72% for tomato and 100% for eggplant), reciprocal micrografting of tomato/eggplant and eggplant/tomato reached the highest success rate (83%) on MS medium supplemented with 30 g L-1 sucrose. Grafted plants should be cultured under the illumination conditions of a 16 h light/8 h dark cycle for optimal growth and quality.
Effects of Salinity Stress on the Growth, Physiology, and Yield of Soybean (Glycine max (L.) Merrill)

Nguyen Thi Linh, Le Thi Tuyet Cham, Vu Ngoc Thang

Received: Dec 08, 2020 / Published: Aug 10, 2021
This study was conducted to evaluate the salt tolerance ability of two soybean cultivars under salinity conditions using a selection of growth and physiological parameters. Three weeks after germination, plants began being treated with either a 100 mM concentration of NaCl in Hoagland solution or a non-salinity (0 mM NaCl) solution. The results showed that the plant height, number of leaves, leaf area index, and shoot and root biomasses were significantly reduced under salt conditions. A similar tendency was observed in several physiological parameters (SPAD, Fv/Fm). Since all the salt-treated plants of the D8 cultivar were dead after 40 days under the 100 mM NaCl treatment, the yield of D8 could not be obtained, while the treated plants of the D140 cultivar experienced a marked decrease in yield compared to the control plants. In this study, we identified that the D140 soybean cultivar had a better salt tolerance than the D8 soybean cultivar at a 100 mM NaCl concentration.
In-vitro Culturability of Soybean Cultivars test

Vu Thi Thuy Hang, Hoang Duc Nhat Linh, Dinh Truong Son, Le Thi Tuyet Cham

Received: Mar 25, 2024 / Accepted: Aug 12, 2024 / Published: Jun 28, 2024
In-vitro regeneration of soybean is a prerequisite for successful genetic transformation. This study aimed to investigate the culturability of three soybean cultivars for in-vitro regeneration, focusing on genotypes, media, explant types, and plant growth regulators. Shoot tips, cotyledons, and hypocotyl segments were excised from 7-day-old in-vitro seedlings as explants for subsequent in-vitro regeneration. All cultivars exhibited good callus formation from all types of explants, with shoot tips and cotyledons being more suitable. Calluses were induced on Gamborg B5 or Murashige and Skoog (MS) media using various concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) or 2,4-dichlorophenoxyacetic acid (2,4D). The combination of BA and TDZ or BA and 2,4D exerted an inhibitory effect on shoot and root induction. Media supplemented with BA produced calluses at a rate of 100%. Appropriate BA concentrations for shoot induction for ĐT35 and VNUAĐ2 were 1.0-2.0 mg L-1, and 2.0-3.0 mg L-1, respectively. MS + 2 mg L-1 BA for shoot tip explants and MS + 3.0 mg L-1 BA for cotyledon explants were appropriate to induce calluses and shoots. As BA concentrations increased, the root induction rate gradually decreased considerably. For both ĐT35 and VNUAĐ2, MS + 2.0 mg L-1 BA + 0.5 or 1.0 mg L-1 αNAA could be used to multiply calluses. Although shoots induced from calluses of ĐT35 were observed on MS + 1.5 or 2.0 mg L-1 BA + 1 mg L-1 αNAA, no shoots were observed for VNUAĐ2.
Effect of Waterlogging Duration on Germination, Physiological Characteristics, and Yield of Mungbean (Vigna radiata L.)

Vu Tien Binh, S. K. Tai Sorgan

Received: Feb 27, 2020 / Published: Jun 18, 2021
The study was conducted to evaluate the germination, physiological responses, yield-related traits, and seed yield of three mungbean varieties, viz. DXVN7, DXVN5, and DX11, under waterlogging coditions in the 2019 Summer. In experiment 1, the seeds of the three mungbean varieties were immersed in distilled water in Petri dishes for 12, 24, 36, 48, and 72h. Afterwards, water was removed and the percentage of germinated seeds was calculated at 84h after sowing. In experiment 2, plants were waterlogged at the seedling stage (25 days after germination) for 3, 6, and 9 days. Waterlogging depth was maintained at 3cm above the soil surface. Physiological traits were determined at the recovery period after termination of waterlogging (45 days after germination). The results showed that waterlogging significantly decreased germination percentages, plant height, root dry weight, leaf relative water content (RWC), SPAD value, Fv/Fm index, leaf photosynthesis, total dry weight, and seed yield of all varieties. Germination percentages at 12 and 24h of waterlogging were not significantly affected, whereas germination was significantly reduced at up to 36h of waterlogging. Seventy-two hours of waterlogging caused failure in germination. Nine days of waterlogging at the seedling stage adversely affected the physiological traits and seed yield of the mungbean varieties with 31% of yield reduction. Meanwhile, plants grew better at 3 days of waterlogging. Among the three varieties, DXVN7 showed the best adaptability under waterlogging conditions, attaining the highest seed germination and yield.
Agronomic Characteristics, Anthocyanin Content, and Antioxidant Activity of Anthocynins Extracted from the Seeds of Black Rice Accessions

Phan Thi Thuy, Nguyen Quoc Trung, Do Thi Huong

Received: Jan 08, 2019 / Published: Feb 13, 2019
The objectives of this study were to investigate the morphological characteristics, grain yield, and anthocyanin content of 36 black rice accessions that were collected from different locations in Vietnam. The results showed that the black rice accessions varied in growth duration (130 to 150 days), plant height (91.5 to 143.6 cm), morphological characteristics, and yield components. Grain yield of the black rice accessions ranged from 2.8 to 8.7 tons ha-1. The black rice accessions were classified into four groups based on their anthocyanin content: group I > 0.1% anthocyanin, group II 0.05%-0.1%, group III 0.001%-0.05%, and group IV < 0.001%. BR7 had the highest anthocyanin content (0.1438%), followed by BR5 (0.1317%). Anthocyanins with the strongest antioxidant activities were extracted from BR8, BR35, BR6, BR27, BR30, BR32, BR18, BR17, BR19, and BR1 with IC50 values less than 2 µg mL-1. Seven promising black rice accessions, namely BR1, BR14, BR17, BR25, BR30, BR34, and BR35, were selected for further research based on their high anthocyanin contents, and good grain yield and yield components.
Nitrogen-Use Efficiency Evaluation and Genome Survey of Vietnamese Rice Landraces (Oryza sativa L.)

Hanh Thi Thuy Nguyen, Thuy Linh Mai Dinh, Trung Quoc Nguyen, Cuong Van Pham

Received: Oct 19, 2018 / Accepted: Oct 22, 2018 / Published: Oct 22, 2018
The overuse of fertilizers can result in many adverse effects such as decreasing fertilizer use efficiency of plants, wasting resources, increasing farming costs, and polluting our environment. Local rice landraces including indigenous and local rice varieties, may contain considerable genetic diversity that can serve as sources of germplasm for genetic improvements of nutrient use efficiency, yield, resistance to pests and pathogens, and important agronomic traits. Increasing the fertilizer use efficiency of crops by developing new rice varieties is necessary for sustainable agriculture. In this study, six rice varieties, Chiem Tay (CT), Te Tep (TT), Re Bac Ninh (RB), IR24, P6DB, and Khang Dan 18 (KD18), were evaluated for nitrogen use efficiency. Two landraces, P6DB and CT, which showed the lowest and highest values of nitrogen use efficiencies, were selected for a genome survey. Ninety-seven out of the 1051 surveyed markers indicated polymorphisms. These polymorphic markers were distributed along to each of the 12 chromosomes and were either scattered quite evenly on a chromosome or were condensed at particular regions in the physical map. The obtained information on nitrogen use efficiency (NUE) variation and the marker map should be very useful to further identify QTLs/genes involving in NUE as well as other genetic analyses toward the development of sustainable agriculture.
The Influence of Bulb Storage Regimes on the Growth and Flowering of Hippeastrum (Hippeastrum hybridum Hort.)

Huyen Trang Thi Pham, Nam Van Dinh, Thanh Nga Thi Trinh, Thu Ha Thi Phung, Thuy Hanh Thi Nguyen, Hoa Hanh Nguyen

Received: Oct 19, 2018 / Accepted: Oct 22, 2018 / Published: Oct 22, 2018
Hippeastrum hybridum cultivar ‘Cam Tu’ bulbs were subjected to ten different treatment regimens to evaluate the effect of temperature (4°C or 25°C), duration (4, 6, or 8 weeks), and wrapping materials (coir fiber or newspaper) on their growth and flowering. The data revealed that the storage treatments had significant effects on several growth and flowering characteristics of H. hybridum cv. ‘Cam Tu’. In terms of vegetative characteristics, bulbs stored at 4°C with coir fiber wrapping for 6 weeks (T9) had the largest leaf size (a length of 39.46 cm, a width of 4.39 cm), the longest flower scape (48.64 cm in length), and the longest pedicel (4.75 cm in length). Bulbs of the T9 treatment also showed the shortest time to flower bud emergence (62.24 days) and first flower opening (80.43 days), and the date of 70% first flower fully opening was January 19, 2018. Both of the two thermal treatment regimens shortened flowering time (80.43-103.16 days) compared to the control (132.46 days). However, the number of bulblets per plant, number of leaves per plant, number of florets per scape, floret diameter, longevity of a flower, and longevity of a flower scape were not statistically impacted by the treatments.
Optimization of Chlorogenic Acid Extraction from Green Coffee Beans Using Response Surface Methodology

Lai Thi Ngoc Ha, Nguyen Viet Phuong, Tran Thi Hoai, Dao Thi Viet Ha, Hoang Hai Ha

Received: Apr 10, 2019 / Published: Jun 06, 2019
Chlorogenic acid is a natural antioxidant that is widespread in the plant kingdom and can be found at a high content level in green coffee beans. This secondary metabolite in green coffee beans has potent biological properties including antioxidant, anti-inflammatory, anti-cancer, anti-obesity, anti-hypertension, and anticonvulsant. In this study, the extraction of chlorogenic acid from Vietnamese green coffee beans was optimized using the response surface methodology. A second-order polynomial model with three important variables (liquid-to-solid ratio, temperature, and extraction time) was used. A rotatable central composite design consisting of 21 experimental runs with three replicates at the center point was applied to describe the experimental data. The experimental results properly conformed to the constructed model (R2 = 0.8549). The optimized conditions were as follows: 40% ethanol (v/v), a liquid-to-solid ratio of 11.77, at 85oC for 64 min. Four extractions were performed in parallel using the optimal conditions to validate the model. The experimental values highly agreed with the predicted value (P <0.05).
Host Genotype and Edaphic Factors Cumulatively Influence the Occurrence of Siderophore-producing Bacteria Associated with Rice (Oryza sativa L.)

Phan Thi Thuy, Chung Wei-Ching, Young Li-Sen

Received: Apr 05, 2021 / Published: Mar 30, 2022
Seed-borne rice endophytes are capable of disseminating into host plant tissues as well as to their rhizosphere. Here, we investigated the occurrence of siderophore-producing bacteria (SPB) in the seed endospheres of two distinct rice (Oryza sativa L.) cultivars, TK8 (ssp. japonica) and TCN1 (ssp. indica), and their dissemination into the rhizospheres through culture-dependent methods. Their patterns of occurrence in the rhizospheres as well as in the root and shoot tissues of 30 day-old cultivars grown in three different kinds of soils were tested. The significance of SPB on Fe sequestration of TCN1 was studied using Enterobacter sp. LS-756. TK8 seeds were found to be not only abundant in endopsheric SPB (> 10-fold), but also exhibited enhanced SPB dissemination into the rhizosphere (1.3-fold) as compared to TCN1. The proportion of endophytic SPB was consistently higher in roots than in shoots, and it was found to decline with decreasing soil pH. A similar declining trend was further evident through the analysis of SPB composition in the rhizospheric and bulk soils. LS-756-inoculated TCN1 seedlings under low availability of Fe showed 32%, 178%, and 368% increases in Fe, chlorophyll, and chlorophyll b contents as compared to the uninoculated controls. Thus, the occurrence of seed-borne endophytic SPB and their dissemination into the rhizosphere vary significantly according to the rice genotype. Higher co-occurrence of SPB in the rhizosphere and internal root tissues of rice plants grown under Fe-limited conditions and the enhanced Fe uptake due to SPB inoculation substantiated their potential involvement in Fe sequestration.
Effects of Plant Growth Regulators and Sucrose on the Regeneration of Paphiopedilum micranthum var. North Vietnam

Binh Tien Vu, S. K. Tai Sorgan

Received: Aug 20, 2018 / Accepted: Aug 20, 2018 / Published: Aug 20, 2018
The objective of this study was to determine the effects of 2,4-Dichlorophenoxyacetic acid (2,4-D) combined with 6-Benzylaminopurine (BAP) and sucrose on the shoot and root regeneration of Paph. micranthum var. North Vietnam. Young fresh leaves were cut into small explants and then cultured on media containing 2,4-D (0.5, 1.0, or 2.0 ppm). Within each of the 2,4-D concentration, BAP was added at three different concentrations (0.5, 1.0, or 2.0 ppm) for callus induction after 4 weeks. Shoot tips (2-3 mm in length) from the leaf-derived calluses were then transferred vertically on half-strength MS medium (2,4-D combined with BAP (0.5, 1.0, or 2.0 ppm) for shooting, and rootless shoots with 2 leaves were transferred to the same MS medium for rooting after 4 months of culture. Rootless shoots with 2 leaves were also transferred vertically on half-strength MS medium with different sucrose concentrations (0, 10, 20, 30, or 40 g L-1) to optimize the growth and development of the plantlets. The results indicated that the optimum degree of shoot and root regeneration occurred on half-strength MS medium containing 2.0 ppm 2,4-D combined with 2.0 ppm BAP. Approximately 86.6% of the cultures responded with 3.3 shoots and 3.0 leaves/explant. In addition, under the optimal concentrations, the number and length of roots per explant after 4 months of culture were 2.1 roots per explant and an average root length of 1.1 cm. MS medium supplemented with 30 g L-1 sucrose had the highest number of leaves and roots, and also the longest average length of roots, which gave 3.8 leaves, 3.3 roots/explant, and a root length of 1.8 cm after 4 months.
Effects of Osmotic Stress Induced by Peg and NaCl on the Germination and Early Growth of Mung Bean

Thang Ngoc Vu, Khuynh The Bui, Gioi Huy Dong, Tuan Anh Tran, Tuyet Cham Thi Le, Chinh Dinh Vu

Received: Oct 15, 2018 / Accepted: Oct 19, 2018 / Published: Oct 22, 2018
This study was performed to evaluate the effects of osmotic stress (measured by different water potentials) induced by Polyethylene Glycol 6000 (PEG) and NaCl solutions at the germination stage of five mung bean varieties (DX11, DX208, DX14, DX17, and DX22). Five water potentials: 0 (control), -0.15, -0.49, -1.03, and -1.76 Mpa were used as treatments in this study. The germination rates, root and shoot lengths, root and shoot fresh weights, and dry weights of the plants were measured. The results showed that the germination rate of the mung bean varieties decreased with increased NaCl or PEG concentrations. The germination rates of the mung bean varieties in the PEG treatments were higher than those in the NaCl treatments. In addition, low water potentials induced by NaCl (-1.76 Mpa and -1.03 Mpa) inhibited germination and seeding growth of all the mung bean varieties. The growth parameters of the mung bean seedlings, such as root and shoot lengths, fresh weights of roots and shoots, and plant dry weights, were reduced under low water potentials treated with either PEG or NaCl. However, more severe damage in seedling growth was observed in the NaCl induced treatments. Amongst five mung bean varieties used in this study, DX17 was more drought and salt tolerant than the other mung bean varieties.

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