Optimal Culture Conditions for the Enhanced Mycelial Growth and Cultivation of Shiitake Mushroom (Lentinula edodes)

Date Received: May 24, 2023

Date Published: Dec 29, 2023

DOI: https://doi.org/10.31817/vjas.2023.6.4.06

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Issue: Vol. 6 No. 4 (2023): Vietnam Journal of Agricultural Sciences

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ENGINEERING AND TECHNOLOGY

How to Cite:

Trang, N., Thuy, N., Mo, N., Luyen, N., & Nghien, N. (2023). Optimal Culture Conditions for the Enhanced Mycelial Growth and Cultivation of Shiitake Mushroom (Lentinula edodes). Vietnam Journal of Agricultural Sciences, 6(4), 1958–1968. https://doi.org/10.31817/vjas.2023.6.4.06

Optimal Culture Conditions for the Enhanced Mycelial Growth and Cultivation of Shiitake Mushroom (Lentinula edodes)

Nguyen Thi Huyen Trang (*) 1 , Nguyen Thi Bich Thuy 2 , Nguyen Thi Mo 1 , Nguyen Thi Luyen 2   , Ngo Xuan Nghien 2

  • Corresponding author: ntbthuy.cnsh@vnua.edu.vn
  • 1 Institute for Edible and Medicinal Mushrooms Research and Development, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Institute for Edible and Medicinal Mushrooms Research and Development, Vietnam National University of Agriculture, Hanoi 131000, Vietnam; Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam

    • Keywords

    Shiitake, Lentinula edodes, cultivation, mushroom

    Abstract


    Lentinula edodes (Berk.) Pegler, commonly known as shiitake mushroom, is one of the most popular edible mushrooms with valuable medicinal properties. Despite its global popularity, the cultivation of shiitake in Vietnam has been limited due to a lack of research on this macrofungus. This study aimed to optimize the culture conditions for the mycelial growth and fruiting body development of two shiitake strains, J1 and J2. The culture conditions were optimized by one-factor-at-a-time method. A temperature of 25°C was found to be optimal for the growth of both strains, while glucose was identified as the most effective carbon source. Yeast extract and ammonium chloride (NH4Cl) were found to be the optimal nitrogen sources for favorable mycelial growth of strains J1 and J2, respectively. The most suitable spawning material for upscaling the mycelium was a mixture of 74% grain rice, 25% sawdust, and 1% CaCO3. Additionally, the cultivation of strain J2 on a mixture of 69% corncobs, 20% sawdust, 10% wheat bran, and 1% CaCO3 led to a higher biological yield (36.5%) than that of strain J1 (23.5%). These findings provide useful information for improving the cultivation technology of shiitake and expanding its cultivation in Vietnam.

    References

    Alberti M. M., Mejía S. J., Pérez-Chávez A. M., Lio V. & Albertó E. (2022). Effects of incubation time and “browning” on yield and proximate composition of the edible mushroom Lentinula edodes. Brazilian Archives of Biology and Technology. 65: 1-10.

    Ashrafuzzaman M., Kamruzzaman A.K.M., Razi Ismail M., Shahidullah S.M. & Fakir S.A. (2009). Substrate affects growth and yield of shiitake mushroom. African Journal of Biotechnology. 8: 2999-3006.

    Bisen P. S., Baghel R. K., Sanodiya B. S., Thakur G. S. & Prasad G. B. K. S. (2010). Lentinus edodes: A macrofungus with pharmacological activities. Current Medicinal Chemistry. 17: 2419-2430.

    Bisko N., Mustafin K., Al-Maali G., Suleimenova Z., Lomberg M., Narmuratova Z., Mykchaylova O., Mytropolska N. & Zhakipbekova A. (2020). Effects of cultivation parameters on intracellular polysaccharide production in submerged culture of the edible medicinal mushroom Lentinula edodes. Czech Mycology. 72(1): 1-17.

    Chen L., Gong Y., Cai Y., Liu W., Zhou Y., Xiao Y., Xu Z., Liu Y., Lei X., Wang G., Guo M., Ma X. & Bian Y. (2016). Genome sequence of the edible cultivated mushroom Lentinula edodes (shiitake) reveals insights into lignocellulose degradation. PLoS ONE. 11(8): 1-20.

    Curvetto N., Figlas D. & Delmastro S. (2002). Sunflower seed hulls as substrate for the cultivation of shiitake mushrooms. HortTechnology. 12(4): 652-655.

    Thuy D. T. T & Hieu N. V. (2020). Structure changing in maize-based agriculture production in Vietnam: Case Study in Son La Province. Sustainable Environment Agricultural Science. 4(1): 65-76.

    Deshaware S., Marathe S. J., Bedade D., Deska J. & Shamekh S. (2021). Investigation on mycelial growth requirements of Cantharellus cibarius under laboratory conditions. Archives of Microbiology. 203(4): 1539-1545.

    Dissasa G. (2022). Cultivation of different Oyster Mushroom (Pleurotus species) on coffee waste and determination of their relative biological efficiency and pectinase enzyme production, Ethiopia. International Journal of Microbiology. 5: 5219939. DOI: 10.1155/2022/5219939.

    Dos Santos I. R., Mohamed T. A., Borges L. L., Nahas H. A., Abdel-Azeem M. A., Carvalho C., Bezerra J. P., Gupta V. K., Xavier-Santos S., Mossa M. I. & Abdel-Azeem A. M. (2022). The global tendency in the research of biological activity in endophytic fungi: A scientometric analysis. Current Research in Environmental and Applied Mycology. 12(1): 1-14.

    Fukushima-Sakuno E. (2020). Bioactive small secondary metabolites from the mushrooms Lentinula edodes and Flammulina velutipes. Journal of Antibiotics. 73(10): 687-696.

    Gonkhom D., Luangharn T., Hyde K. D., Stadler M. & Thongklang N. (2022). Optimal conditions for mycelial growth of medicinal mushrooms belonging to the genus Hericium. Mycological Progress. 21(9): 82. DOI: 10.1007/s11557-022-01829-6.

    Ishikawa N. K., Kasuya M. C. M. & Dantas Vanetti M. C. (2001). Antibacterial activity of Lentinula edodes grown in liquid medium. Brazilian Journal of Microbiology. 32(3): 206-210.

    Itoo Z. A. & Reshi Z. A. (2014). Effect of different nitrogen and carbon sources and concentrations on the mycelial growth of ectomycorrhizal fungi under in-vitro conditions. Scandinavian Journal of Forest Research. 29(7): 619-628.

    Koo J., Lee S., Lee S., Park W., Hwang J. S. & Kim M. (2018). Browning technology for shiitake in sawdust using LED source. Journal of Mushrooms. 16(4): 331-333.

    Krupodorova T. A., Barshteyn V. Y., Kizitska T. O. & Pokas E. V. (2019). Effect of cultivation conditions on mycelial growth and antibacterial activity of Lentinula edodes and Fomitopsis betulina. Czech Mycology. 7(12): 167-186.

    Krupodorova T.A., Barshteyn V.Y. & Sekan A.S. (2021). Review of the basic cultivation conditions influence on the growth of basidiomycetes. Current Research in Environmental and Applied Mycology. 11(1): 491-531.

    Kumar V., Kaur M., Varunesh K. C. & Mishra S. (2019). Effect of different media, temperature and pH on radial mycelial growth of Lentinula edodes strain Le-17-04. Journal of Pharmacognosy and Phytochemistry. 8(1): 345-348.

    Kwon J. K., Kim, S. H., Jeon J. G., Kang Y. K. & Jang K. (2018). Development of environmental control system for high-quality shiitake mushroom (Lentinus edodes (Berk.) Sing.) production. Journal of Biosystems Engineering. 43(4): 342-351.

    Li J., Duan Y., Hu Z., Yang F., Wu X. & Zhang R. (2022). Physiological mechanisms by which gypsum increases the growth and yield of Lentinula edodes. Applied Microbiology and Biotechnology. 106(7): 2677-2688.

    Miles P. G. & Chang S.-T. (1997). Mushroom Biology: Concise Basics and Current Developments. In P. G. Miles (Ed.), Mushroom Biology: Concise Basics and Current Developments. World Scientific Publishing Company: 41.

    Nguyen B. T. T., Le V. Van, Nguyen H. T. T., Nguyen L. T., Tran A. D. & Ngo N. X. (2021). Successful rescue of wild Trametes versicolor strains using sawdust and rice husk-based substrate. Pakistan Journal of Biological Sciences. 24(3): 374-382.

    Nguyen B. T. T., Ngo N. X., Le V. V., Nguyen L. T., Kana R. & Nguyen H. D. (2019). Optimal culture conditions for mycelial growth and fruiting body formation of Ling Zhi mushroom Ganoderma lucidum strain GA3. Vietnam Journal of Science, Technology and Engineering. 61(1): 62-67.

    Nguyen B. T. T., Le V.V., Nguyen H. T. T., Nguyen L. T., Tran T. T. T. & Ngo N. X. (2021). Nutritional requirements for the enhanced mycelial growth and yield performance of Trametes versicolor. Journal of Applied Biology and Biotechnology. 9(1): 1-7.

    Nguyen L. T., Le, V. V, Nguyen B. T. T., Nguyen H. T. T., Tran A. D. & Ngo N. X. (2023). Optimization of mycelial growth and cultivation of wild Ganoderma sinense. Biotechnologia. 104(1): 65-74.

    Nguyen Thi Luyen, Nguyen Thi Bich Thuy, Tran Dong Anh, Khong Thi Kim Tien, Tran Thi Thuy Trang, Nguyen Thi Mo, Le Van Ve, Nguyen Thi Huyen Trang. Effect of cultivation substrates on mycelial growth and fruiting body formation of Lentinula edodes. Journal of Vietnam Agricultural Science and Technology. 113(4): 49-55 (in Vietnamese).

    Ribas L. C. C., de Mendonça M. M., Camelini C. M. & Soares C. H. L. (2009). Use of spent mushroom substrates from Agaricus subrufescens (syn. A. blazei, A. brasiliensis) and Lentinula edodes productions in the enrichment of a soil-based potting media for lettuce (Lactuca sativa) cultivation: Growth promotion and soil bioremediati. Bioresource Technology. 100(20): 4750-4757.

    Thakur, M. P. (2020). Advances in mushroom production: Key to food, nutritional and employment security: A review. Indian Phytopathology. 73(3): 377-395.

    Yu H., Zhang D., Zhang L., Li, Q., Song C., Shang X., Bao D., Tan Q., Chen H. & Lv B. (2022). Corncob as a substrate for the cultivation of Lentinula edodes. Waste and Biomass Valorization. 13(2): 929-939. DOI: 10.1007/s12649-021-01575-y.

    Zervakis G., Philippoussis A., Ioannldou S. & Dlamantopoulou P. (2001). Mycelium growth kinetics and optimal temperature conditions for the cultivation of edible mushroom species on lignocellulosic substrates. Folia Microbiologica. 46(3): 231-234.

    Zhang M., Zhang Y., Zhang L. & Tian Q. (2019a). Mushroom polysaccharide lentinan for treating different types of cancers: A review of 12 years clinical studies in China. In Progress in Molecular Biology and Translational Science (1st ed., Vol. 163). Elsevier Inc.

    Zhang W. R., Liu S. R., Kuang Y. B. & Zheng S. Z. (2019b). Development of a novel spawn (block spawn) of an edible mushroom, Pleurotus ostreatus, in liquid culture and its cultivation Eevaluation. Mycobiology. 47(1): 97-104.